Thiago GONCALVES

Lignin Oxidative Fungal Enzymatic Response

  • Supervisor: David Cannella
  • Research center: Laboratoire de Prodsuction et de Biostimulation des Plantes cultivées (CPBL)
  • Research start date: 15.11.2021

Description

One third of agricultural production is lost yearly due to consumption of crops by phytopathogens, which can be reduced with Science help. To overcome increasing resistance of pathogens to phytopharmaceuticals new active compounds are needed. This project was designed to provide such new class of phytopharmaceutical molecules. Lignin derived nanoparticles and photo-active phenols possess microbicide activity totally compatible with plant growth and sustainable environmental requirements. The goal of this proposal is to source in wastes of industrial lignocellulosic biorefineries lignin pools to achieve a large spectrum phytopharmaceutical. Lignin will be modified via redox enzymes through novel bioprocessing and the effectiveness of their phytoprotection assessed on medium-scale trial.

Biography

Thiago Gonçalves holds a Bachelor of Science in Biochemistry from the Federal University of Viçosa (UFV) and PhD in Functional and Molecular Biology from the State University of Campinas (UNICAMP). He worked in respected research centers, such as the Biotechnology Applied to Agriculture Institute (BIAGRO, Viçosa/Brazil), and Brazilian Biorenewables National Laboratory (LNBR/CNPEM, Campinas/Brazil). His research background goes through 1) Production, purification and characterization of lignocellulolytic enzymes and its applications in human non-digestible sugar modification, saccharification of plant biomass and cellulosic pulp bleaching for the paper industry; 2 Hheterologous production of enzymes in bacteria and filamentous fungi, which were selected based on new generation omics approaches, for application in the bioenergy and bioproducts fields; 3) Bioconversion of lignin derived molecules in high value chemicals, mainly in the conversion of ferulic acid in the aromatic compounds vanillin and eugenol, with high appeal in the food industry; 4) The understanding of the role of fungi oxidative enzymes in plant derived carbohydrates and phenolics modification, in order to apply this knowledge in the improvement of yield of food crops.

Publications

  1. Melanin, a fungal photosensitizer for cellulose oxidizing AA9-LPMO enzymes. Monclaro, A.; Gonçalves, T.; Magri, S.; Ovaert, J.; Decembrino, D.; Debecker, D.P.; Kadowaki, M.; Doneux, T.; De Leener, G.; Zarattini, M.; Luhmer, M.; Cannella, D. ChemCatChem, v.15, e202300602, p. 1-8, (2023).
  2. Integrative omics analyses of the ligninolytic Rhodosporidium fluviale LM-2 disclose catabolic pathways for biobased chemical production. Vilela, N.; Tomazetto, G.; Gonçalves, T.A.; Sodré, V.; Persinoti, G.F.; Moraes, E.C.; de Oliveira, A.H.C.; da Silva, S.N.; Fill, T.P.; Damasio, A.; Squina, F.M. Biotechnology for Biofuels and Bioproducts, v. 16, article number 5, p. 1-17, (2023).
  3. Recombinant expression, purification and characterization of an active bacterial feruloyl-CoA synthase with potential for application in vanillin production. dos Santos, O.A.L.; Gonçalves, T.A.; Sodré, V.; Vilela, N.; Tomazetto, G.; Squina, F.M. ; Garcia, W. Protein Expression and Purification, v. 197, 106109, p 1-7, (2022).
  4. Oxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases. Franco Cairo, J.P.; Mandelli, F.; Tramontina, R.; Cannella, D.; Paradisi, A.; Ciano, L.; Ferreira, M.R.; Liberato, M.V.; Brenelli, L.B.; Gonçalves, T.A.; Rodrigues, G.N.; Alvarez, T.M.; Mofatto, L.S.; Carazzolle, M.F.; Pradella, J.G.C.; Paes Leme, A.F.; Costa-Leonardo, A.M.; Oliveira-Neto, M.; Damásio, A.; Davies, G.J.; Felby, C.; Walton, P.H.; Squina, F.M.  Green Chemistry, v. 24, p. 4845-4858, (2022).
  5. Applying biochemical and structural characterization of hydroxycinnamate catabolic enzymes from soil metagenome for lignin valorization strategies. Gonçalves, T.A.; Sodré, V.; da Silva, S.N.; Vilela, N.; Tomazetto, G.; Araujo, J. N.; Muniz, J.R.C.; Fill, T.P.; Damásio, A.; Garcia, W.; Squina, F.M. Applied Microbiology and Biotechnology, v. 106, p. 2503-2516, (2022).
  6. Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600. de Figueiredo, F.L.; de Oliveira, A.C.P.; Terrasan, C.R.F.; Gonçalves, T.A.; Gehardt, J.A.; Tomazetto, G.; Persinoti, G. F.; Rubio, M.V.; Pena, J.A.T.; Araújo, M.F.; de Carvalho Silvello, M.A.; Franco, T.T.; Rabelo, S.C.; Goldbeck, R.; Squina, F.M.; Damasio, A. Biotechnology for Biofuels, v. 14, article number 96, p. 1-17, (2021).
  7. Microbial enrichment and meta-omics analysis identify CAZymes from mangrove sediments with unique Properties. Paixão, D.A.A.; Tomazetto, G.; Sodré, V.R.; Gonçalves, T.A.; Uchima, C.A.; Büchli, F.; Alvarez, T.M.; Persinoti, G.F.; da Silva, M.J.; Bragatto, J.; Liberato, M.V.; Cairo, J.P.L.F.; Paes Leme, A.F.; Squina, F.M. Enzyme and Microbial Technology, v. 148, 109820, p. 1-12, (2021).
  8. On the roles of AA15 lytic polysaccharide monooxygenases derived from the termite Coptotermes gestroi. Franco Cairo, J.P.L.; Cannella, D.; Oliveira, L.C.; Gonçalves, T.A.; Rubio, M.V.; Terrasan, C.R.F.; Tramontina, R.; Mofato, L.S.; Carazzolle, M.F.; Garcia, W.; Felby, C.; Damásio, A.; Walton, P.H.; Squina F.  Journal of Inorganic Biochemistry, v. 216, 111316, p. 1-12, (2021).
  9. Insights into the dual cleavage activity of the GH16 laminarinase enzyme class on β-1,3 and β-1,4 glycosidic bonds. Liberato, M.V.; Teixeira Prates, E.; Gonçalves, T.A.; Bernardes, A.; Vilela, N.; Fattori, J.; Ematsu, G.C.; Chinaglia, M.; Machi Gomes, E.R.; Migliorini Figueira, A.C.; Damásio, A.; Polikarpov, I.; Skaf, M.R S.; Squina, F.M. Journal of Biological Chemistry, v. 296, 100385, p. 1-13, (2021).
  10. Functional characterization of a novel thermophilic exo-arabinanase from Thermothielavioides terrestris. Velasco, J.; Oliva, B.; Gonçalves, A.L. ; Lima, A.S. ; Ferreira, G.; Franca, B.A.; Mulinari, E.J.; Gonçalves, T.A.; Squina F.M. ; Kadowaki, M.; Maiorano, A.; Polikarpov, I.; Oliveira, L.C. ; Segato, F. Applied Microbiology and Biotechnology, v. 104, p. 8309-8326, (2020).
  11. The structure of a prokaryotic feruloyl-CoA hydratase-lyase from a lignin-degrading consortium with high oligomerization stability under extreme pHs. Liberato, M.V.; Araújo, J.N.; Sodré, V.; Gonçalves, T.A.; Vilela, N.; Moraes, E.C.; Garcia, W.; Squina, F.M. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, v. 1868(3), 140344, p. 1-8, (2020).
  12. Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain. Brenelli, L.B.; Persinoti, G. F.; Cairo, J.P.L.F.; Liberato, M.V.; Gonçalves, T.A.; Otero, I.V.R.; Mainardi, P.H.; Felby, C.; Sette, L.D.; Squina, F.M. Scientific Reports, v. 9, article number 17564, p. 1-15, (2019).
  13. Heterologous expression and functional characterization of a GH10 endoxylanase from Aspergillus fumigatus var. niveus with potential biotechnological application. Velasco, J.; Oliva, B.; Mulinari, E.J.; Quintero, L.P.; Lima, A.S.; Gonçalves, T.A.; Damásio, A.R.L.; Squina, F.M.; Milagres, A.M.F.; Abdella, A.; Wilkins, M. R.; Segato, F. Biotechnology Reports, v. 24, e00382, p. 1-10, (2019).
  14. The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures. Valadares, F.; Gonçalves, T.A.; Damásio, A.R.L.; Milagres, A.M.F.; Squina, F.M.; Segato, F.; Ferraz, A. Enzyme and Microbial Technology, v130, 109370, p. 1-10, (2019).
  15. An alkaline active feruloyl-CoA synthetase from soil metagenome as a potential key enzyme for lignin valorization strategies. Sodré, V.; Araujo, J.N.; Gonçalves, T.A.; Vilela, N.; Braz, A.S.K.; Franco, T. T.; De Oliveira Neto, M.; Damásio, A.R.L.; Garcia, W.; Squina, F.M. PLoS One v. 14, e0212629, p. 1-21, (2019).
  16. Comparison of the production of enzymes to cell wall hydrolysis using different carbon sources by Penicillium echinulatum strains and its hydrolysis potential for lignocelullosic biomass. Schneider, W.D.H.; Gonçalves, T.A.; Uchima, C.A.; Reis, L.; Fontana, R. C.; Squina, F.M.; Dillon, A.J. P.; Camassola, M. Process Biochemistry, v. 66, p. 162-170, (2018).
  17. Xyloglucan breakdown by endo-xyloglucanase family 74 from Aspergillus fumigatus. Damásio, A.R.L.; Rubio, M.V.; Gonçalves, T.A.; Persinoti, G.F.; Segato, F.; Prade, R.A.; Contesini, F.J.; de Souza, A.P.; Buckeridge, M.S.; Squina, F.M. Applied Microbiology and Biotechnology, v. 101(7), p. 2893-2903, (2017).
  18. Molecular basis of substrate recognition and specificity revealed in family 12 glycoside hydrolases. Calzado, F.; Prates, E.T.; Gonçalves, T.A.; Rubio, M.V.; Zubieta, M.P.; Squina, F.M.; Skaf, M.S.; Damásio, A.R.L. Biotechnology and Bioengineering, v. 113(12), p. 2577-2586, (2016).
  19. Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass. Schneider, W.D.H.; Gonçalves, T.A.; Uchima, C.A.; Couger, M.B.; Prade, R.A.; Squina, F.M.; Dillon, A.J.P.; Camassola, M. Biotechnology for Biofuels, v. 9, article number 66, p. 1-26, (2016).
  20. Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi. Franco Cairo, J.P.L.; Carazzolle, M.F.; Leonardo, F.C.; Mofatto, L.S.; Brenelli, L.B.; Gonçalves, T.A.; Uchima, C.A.; Domingues, R.R.; Alvarez, T.M.; Tramontina, R.; Vidal, R.O.; Costa, F.F.; Costa-Leonardo, A.M.; Paes Leme, A.F.; Pereira, G.A.G.; Squina, F.M. Frontiers in Microbiology, v. 7, p. 1-21, (2016).
  21. Effect of hemicellulolytic enzymes to improve sugarcane bagasse saccharification and xylooligosaccharides production. Goldbeck, R.; Gonçalves, T.A.; Damásio, A.R.L.; Brenelli, L.B.; Wolf, L.D.; Paixão, D.A.A.; Rocha, G.J.M.; Squina, F. M. Journal of Molecular Catalysis. B, Enzymatic, v. 131, p. 36-46, (2016).
  22. Characterization and Low-Resolution Structure of an Extremely Thermostable Esterase of Potential Biotechnological Interest from Pyrococcus furiosus. Mandelli, F.; Gonçalves, T.A.; Gandin, C.A.; Oliveira, A.C.P.; de Oliveira Neto, M.; Squina, F.M. Molecular Biotechnology, v. 58, p. 757-766, (2016).
  23. Exploring glycoside hydrolases and accessory proteins from wood decay fungi to enhance sugarcane bagasse saccharification. Valadares, F.; Gonçalves, T.A.; Gonçalves, D.S.P.O.; Segato, F.; Romanel, E.; Milagres, A.M.F.; Squina, F.M.; Ferraz, A. Biotechnology for Biofuels, v. 9, article number 110, p. 1-12, (2016).
  24. Development of hemicellulolytic enzyme mixtures for plant biomass deconstruction on target biotechnological applications. Goldbeck, R.; Damásio, A.R.L.; Gonçalves, T.A.; Machado, C.B.; Paixão, D.A.A.; Wolf, L.D.; Mandelli, F.; Rocha, G.J.M.; Ruller, R.; Squina, F.M. Applied Microbiology and Biotechnology, v. 98, p. 8513-8525, (2014).
  25. Development and Biotechnological Application of a Novel Endoxylanase Family GH10 Identified from Sugarcane Soil Metagenome. Alvarez, T.M.; Goldbeck, R.; dos Santos, C.R.; Paixão, D.A.A.; Gonçalves, T.A.; Franco Cairo, J.P.L.; Almeida, R.F.A; Pereira, I.O.; Rocha, G.J.M.; Cota, J.; Büchli, F.; Citadini, A.P.; Ruller, R.; Polo, C.C.; de Oliveira Neto, M.; Murakami, M.T.; Squina, F.M. Plos One, v. 8(7), e70014, p. 1-13, (2013).
  26. Functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides. Gonçalves, T.A.; Damásio, A.R.L.; Segato, F.; Alvarez, T.M.; Bragatto, J.; Brenelli, L.B.; Citadini, A.P.S.; Murakami, M.T.; Ruller, R.; Paes Leme, A.F.; Prade, R.A.; Squina, F.M. Bioresource Technology, v. 119, p. 293-299, (2012).
  27. Two structurally discrete GH7-cellobiohydrolases compete for the same cellulosic substrate fiber. Segato, F.; Damásio, A.R.L.; Gonçalves, T.A.; Murakami, M.T.; Squina, F.M.; Polizeli, M.L.T.M.; Mort, A.; Prade, R.A. Biotechnology for Biofuels, v. 5, article number 21, p.  1-12, (2012).
  28. High-yield secretion of multiple client proteins in Aspergillus. Segato, F.; Damásio, A.R.L.; Gonçalves, T.A.; de Lucas, R.C.; Squina, F.M.; Decker, S.R.; Prade, R.A. Enzyme and Microbial Technology, v. 51, p. 100-106, (2012).

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